AUTORADIOGRAPHIC STUDIES ON THE PROLIFERATION OF ANTIBODY-PRODUCING CELLS IN VITRO
Open Access
- 1 March 1974
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 139 (3), 754-760
- https://doi.org/10.1084/jem.139.3.754
Abstract
A rapid and reliable autoradiographic technique for plaque-forming cells (PFCs) using 14C rather than tritiated thymidine is described. Its application to PFCs developing in vitro shows that (a) practically all PFCs derive from precursors dividing steadily during the culture period, (b) PFC precursors divide in the absence of T-cell helper function, and (c) at least some PFCs may continue to divide.Keywords
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