Solubilization and other studies on adenylate cyclase of baker's yeast
- 1 November 1976
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 159 (2), 363-370
- https://doi.org/10.1042/bj1590363
Abstract
1. Adenylate cyclase of Saccharomyces cerevisiae was sedimented from mechanically disintegrated preparations of yeast over an unusually wide range of centrifugal forces. 2. The enzyme was readily solubilized by Ficoll and by Lubrol PX. Lubrol caused a 2-fold activation. 3. Both particle-bound and Lubrol-solubilized enzyme had an apparent Km for ATP of 1.6 mM in the presence of 0.4 mM-cyclic AMP and 5 mM-MnCl2 at pH 6.2 and 30°C. 4. The Lubrol-solubilized enzyme behaved on gel filtration as a monodisperse protein with an apparent mol.wt. of about 450000.This publication has 17 references indexed in Scilit:
- Quantitative estimation of 3′5′ cyclic AMP phosphodiesterase using anion exchange resin in a batch processAnalytical Biochemistry, 1976
- Studies on cyclic adenosine 3′,5′-monophosphate levels, adenylate cyclase and phosphodiesterase activities in the dimorphic fungus Mucor rouxiiArchives of Biochemistry and Biophysics, 1975
- Dispersion of epinephrine sensitive and insensitive adenylate cyclase from the ciliate Tetrahymena pyriformisBiochimica et Biophysica Acta (BBA) - Enzymology, 1975
- Soluble and membrane‐bound cyclic AMP diesterase activity with a low Michaelis constant in baker's yeastFEBS Letters, 1975
- Demonstration in Aspergillus niger of adenyl cyclase, a cyclic adenosine 3′,5′-monophosphate-binding protein, and studies on intracellular and extracellular phosphodiesterasesCanadian Journal of Microbiology, 1974
- Metabolism of adenosine 3',5'-cyclic monophosphate and induction of fruiting bodies in Coprinus macrorhizus.1973
- Yeast ChromatinEuropean Journal of Biochemistry, 1973
- Adenylate cyclase activity in lubrol-treated membranes from Neurospora crassaBiochimica et Biophysica Acta (BBA) - Enzymology, 1972
- The enzymic composition of the isolated cell wall and plasma membrane of baker's yeastBiochemical Journal, 1970
- A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acidBiochemical Journal, 1956