ISOLATION AND PROPERTIES OF A NUCLEIC ACID HYBRID POLYMERASE
- 1 June 1964
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 51 (6), 1022-1028
- https://doi.org/10.1073/pnas.51.6.1022
Abstract
An enzyme capable of synthesizing DNA-RNA-like homopolymeric hybrids has been isolated from E. coli. The hybrid products are of high molecular weight (sedimentation constant ca. 12), are apparently helical since they exhibit relatively sharp temperature-absorbance curves, and are resistant to RNase and DNase. The reaction is primed by double-stranded polyribonucleotides, polydeoxynucleotides, and DNA. Both chains of the hybrid must be synthesized simultaneously, suggesting that the hybrid polymerase is bifunctional, or dimeric. Single-stranded polynucleotides do not serve as templates.Keywords
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