Blot hybridisation analysis of genomic DNA.
Open Access
- 1 June 1984
- journal article
- review article
- Published by BMJ in Journal of Medical Genetics
- Vol. 21 (3), 164-172
- https://doi.org/10.1136/jmg.21.3.164
Abstract
Restriction endonuclease analysis of specific gene sequences is proving to be a valuable technique for characterisation and diagnosis of inherited disorders. This paper describes detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems and alternatives in the procedure are discussed and a troubleshooting guide has been provided to help rectify faults.Keywords
This publication has 17 references indexed in Scilit:
- The application of DNA recombinant technology to the analysis of the human genome and genetic diseaseHuman Genetics, 1981
- Cloning a cDNA for the pro-alpha 2 chain of human type I collagen.Proceedings of the National Academy of Sciences, 1981
- Model for antenatal diagnosis of β-thalassaemia and other monogenic disorders by molecular analysis of linked DNA polymorphismsNature, 1980
- CONSTRUCTION OF A GENETIC-LINKAGE MAP IN MAN USING RESTRICTION FRAGMENT LENGTH POLYMORPHISMS1980
- Polymorphism of DNA sequence adjacent to human beta-globin structural gene: relationship to sickle mutation.Proceedings of the National Academy of Sciences, 1978
- ANTENATAL DIAGNOSIS OF SICKLE-CELL ANÆMIA BY D.N.A. ANALYSIS OF AMNIOTIC-FLUID CELLSThe Lancet, 1978
- Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized filmFEBS Letters, 1977
- A physical map of the DNA regions flanking the rabbit β-globin geneCell, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Release of Infectious Epstein-Barr Virus by Transformed Marmoset LeukocytesProceedings of the National Academy of Sciences, 1973