Preliminary experience with subcutaneous human ovarian cortex transplantation in the NOD-SCID mouse.
Open Access
- 1 June 1999
- journal article
- Published by Oxford University Press (OUP) in Biology of Reproduction
- Vol. 60 (6), 1462-1467
- https://doi.org/10.1095/biolreprod60.6.1462
Abstract
Xenogeneic transplantation of ovarian cortex into an immunodeficient animal host may be an approach toward fertility preservation for young female patients undergoing cancer therapy. Our objective was to evaluate the development of follicles in human ovarian cortex placed s.c. in non-obese diabetic-severe combined immune deficiency (NOD-SCID) mice (n = 54). The following variables were compared: 1) male versus female mice as hosts, 2) intact versus pituitary down-regulated mice, and 3) warm versus cold tissue transport. After 2 wk, 37 of 50 (74%) of the human xenografts contained follicles. At 12 wk after transplantation, exogenous gonadotropin stimulation resulted in follicle growth in 19 of 37 (51%) of the grafts, including the development of antral follicles, which could be palpated and visualized through the mouse skin. Significantly more developing follicles were identified in male versus female mice (13 of 17 vs. 6 of 20, respectively; p = 0.013) after stimulation. No difference was found between intact and pituitary down-regulated mice as hosts. Follicular survival was significantly increased by warm versus cold tissue transport. Our results suggest that s.c. ovarian cortex xenografting into NOD-SCID mice is feasible. Primordial follicles in ovarian xenografts retain their developmental potential and form antral follicles following gonadotropin stimulation.Keywords
This publication has 22 references indexed in Scilit:
- Allogeneic and Xenogeneic Transplantation of Cryopreserved Ovarian Tissue to Athymic Mice1Biology of Reproduction, 1997
- The Use of SCID Mice in Biotechnology and as a Model for Human DiseaseCritical Reviews in Biotechnology, 1996
- Proliferating Cell Nuclear Antigen Marks the Initiation of Follicular Growth in the Rat1Biology of Reproduction, 1995
- THE EFFECT OF AUTOTRANSPLANTATION OF THE OVARIES TO THE KIDNEYS OR UTERUS ON THE OESTROUS CYCLE OF THE GUINEA-PIGJournal of Endocrinology, 1968
- THE FERTILITY OF MICE WITH ORTHOTOPIC OVARIAN GRAFTS DERIVED FROM FROZEN TISSUEReproduction, 1960
- ORTHOTOPIC OVARIAN TRANSPLANTATION IN MICEJournal of Endocrinology, 1960
- ORTHOTOPIC OVARIAN GRAFTS IN THE GOLDEN HAMSTERJournal of Endocrinology, 1959
- Viability of ovarian tissue after freezingProceedings of the Royal Society of London. B. Biological Sciences, 1957
- Regeneration of rat ovarian tissue grafted after exposure to low temperaturesProceedings of the Royal Society of London. B. Biological Sciences, 1953
- Survival of transplanted ovaries in ratsJournal of Experimental Zoology, 1949