Insolubilized D-Amino Acid Oxidase: Properties and Potential Use
- 1 August 1972
- journal article
- research article
- Published by Taylor & Francis in Separation Science
- Vol. 7 (4), 403-407
- https://doi.org/10.1080/00372367208055582
Abstract
D-Amino acid oxidase has been amide-linked to ω-aminoalkyl agarose particles. The insolubilized enzyme has characteristics similar to the soluble form with respect to stability, pH optimum, affinity for flavin adenine dinucleotide, and general substrate specificity, although smaller Michaelis constants were found for some substrates. The potential application for the separation of the natural L-amino acid isomers from racemates has been demonstrated.Keywords
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