Human Lymphoblastoid Cell Line Producing Specific Antibody against Rh‐Antigen D

Abstract

A lymphoblastoid cell line producing specific anti-Rh antibody against D determinant was established by Epstein-Barr virus (EBV) infection. The lymphocytes were from an immunized male donor with high titer of anti-D antibody. The cells were preselected by rosetting them with Rh-positive erythrocytes before EBV infection. The cell line produced specific antibody of Ig[immunoglobulin]G class, IgG1 subclass, and also nonspecific IgM and IgG. By rerosetting the cell line, the specific antibody-producing population could be enriched and the antibody titer increased in the culture supernatant to the level of immune sera.