Identification of Tn4451 and Tn4452, chloramphenicol resistance transposons from Clostridium perfringens
Open Access
- 1 April 1987
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 169 (4), 1579-1584
- https://doi.org/10.1128/jb.169.4.1579-1584.1987
Abstract
The recombinant plasmids pJIR45 and pJIR97 contain the chloramphenicol resistance determinants derived from the Clostridium perfringens R plasmids pIP401 and pJIR27, respectively. Escherichia coli cultures which harbored these recombinant plasmids rapidly became chloramphenicol sensitive when grown in the absence of chloramphenicol. The loss of resistance was associated with the loss of 6.2-kilobase (kb) segments from both plasmids. Detailed restriction analysis of E. coli- and C. perfringens-derived deletion plasmids indicated that deletion of these segments was essentially precise. Transposition of the 6.2-kb segments was demonstrated by cloning the determinants into a temperature-sensitive plasmid, curing the recombinant plasmids, and selecting chloramphenicol-resistant, plasmid-free clones. Southern hybridization analysis of chromosomal DNA isolated from these recA E. coli clones indicated that the 6.2-kb segments had transposed to different sites on the chromosome. Heteroduplex analysis and restriction mapping indicated that the transposons, Tn4451 (pIP401) and Tn4452 (pJIR27), were closely related and did not contain large inverted or directly repeated sequences. These transposons represent the first transposable elements from the clostridia to be identified and characterized.This publication has 29 references indexed in Scilit:
- Worldwide distribution of the conjugative Clostridium perfringens tetracycline resistance plasmid, pCW3Plasmid, 1985
- Cloning and analysis of the Clostridium perfringens tetracycline resistance plasmid, pCW3Plasmid, 1985
- Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectorsGene, 1985
- Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesisGene, 1983
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- TRANSPOSABLE ELEMENTS IN PROKARYOTESAnnual Review of Genetics, 1981
- Tn554—a site-specific represser-controlled transposon in Staphylococcus aureusNature, 1979
- Amplification of the respiratory NADH dehydrogenase of Escherichia coli by gene cloningGene, 1978
- Characterization and transferability of Clostridium perfringens plasmidsPlasmid, 1977
- A complementation analysis of the restriction and modification of DNA in Escherichia coliJournal of Molecular Biology, 1969