Renaturation of DNA catalysed by yeast DNA repair and recombination protein RAD1O
- 1 February 1992
- journal article
- Published by Springer Nature in Nature
- Vol. 355 (6362), 743-745
- https://doi.org/10.1038/355743a0
Abstract
The RAD10 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of ultraviolet-damaged DNA, and it functions in mitotic recombination. RAD10 has homology to the human excision repair gene ERCC-1. Here we describe the purification of the protein encoded by RAD10 and show that it is a DNA-binding protein with a strong preference for single-stranded DNA. We also show that RAD10 promotes the renaturation of complementary DNA strands.Keywords
This publication has 16 references indexed in Scilit:
- RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination.Molecular and Cellular Biology, 1990
- Characterization of the repressed 5S DNA minichromosomes assembled in vitro with a high-speed supernatant of Xenopus laevis oocytes.Molecular and Cellular Biology, 1988
- Protein complexes formed during the incision reaction catalyzed by theEscherichia coliUvrABC endonucleaseNucleic Acids Research, 1986
- The effect ofEscherichia coliUvr protein binding on the topology of supercoiled DNANucleic Acids Research, 1986
- Enzymatic properties of purified Escherichia coli uvrABC proteins.Proceedings of the National Academy of Sciences, 1983
- A novel repair enzyme: UVRABC excision nuclease of Escherichia coli cuts a DNA strand on both sides of the damaged regionCell, 1983
- Identification of the uvrC gene product.Proceedings of the National Academy of Sciences, 1981
- Recognition of chemical carcinogen-modified DNA by a DNA-binding protein.Proceedings of the National Academy of Sciences, 1980
- Structure at 2.3 Å resolution of the gene 5 product of bacteriophage fd: A DNA unwinding proteinJournal of Molecular Biology, 1979
- Physicochemical properties of DNA binding proteins. Gene 32 protein of T4 and Escherichia coli unwinding proteinBiochemistry, 1975