Skin Sensitizing Antibodies: A Comparative Study of Canine and Human PK and PCA Antibodies and a Canine Myeloma Protein

Abstract

Canine reaginic antibody from 13 atopic animals with hypersensitivity to ragweed pollens has been separated from other canine immunoglobulins, including the γM-globulins, a canine polymeric (10 S) myeloma protein and a canine γG-antibody eliciting a passive cutaneous anaphylactic (PCA) reaction in the guinea pig, by Sephadex G 200 gel filtration and sucrose density gradient ultracentrifugation. The canine reaginic antibody also has been compared with atopic human reaginic antibody, human isohemagglutinins with intermediate sedimentation coefficients, human anti-tetanus toxoid (PCA) antibody and pathologic human immunoglobulins, and has been found to be closely similar in size to the human reaginic antibody. The ability of canine reaginic antisera to elicit a dermal anaphylactic Prausnitz-Küstner reaction was markedly reduced after exposure to 2-mercaptoethanol.