Liquid chromatographic assay of ketoconazole
- 1 January 1981
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 19 (1), 110-113
- https://doi.org/10.1128/aac.19.1.110
Abstract
A reverse-phase, high-pressure liquid chromatographic method for the rapid and quantitative determination of ketoconazole has been developed. Drug levels from 0.5 to 10 microgram/ml can be determined in either yeast nitrogen base medium or human serum by using an octadecylsilane column. A retention time of 4.9 +/- 0.1 min resulted when the drug was eluted from a column with 75% methanol-25% 0.02 M (pH 7.5) phosphate buffer at a flow rate of 2 ml/min. Optimum sensitivity was obtained at a wavelength of 231 nm.This publication has 8 references indexed in Scilit:
- The activity in vitro and in vivo of a new imidazole antifungal, ketoconazoleJournal of Antimicrobial Chemotherapy, 1980
- Ketoconazole, an oral antifungal: laboratory and clinical assessment of imidazole drugsPostgraduate Medical Journal, 1979
- Antimycotic imidazoles. Part 4. Synthesis and antifungal activity of ketoconazole, a new potent orally active broad-spectrum antifungal agentJournal of Medicinal Chemistry, 1979
- Oral Therapy for Experimental Coccidioidomycosis with R41 400 (Ketoconazole), a New Imidazole1,2American Review of Respiratory Disease, 1978
- Rapid determination of serum 5-fluorocytosine levels by high-performance liquid chromatographyAntimicrobial Agents and Chemotherapy, 1978
- Influence of Antioxidants on the Bioactivity of Amphotericin BAntimicrobial Agents and Chemotherapy, 1977
- Quantitation of Amphotericin B with Use of High-Pressure Liquid ChromatographyThe Journal of Infectious Diseases, 1977
- Assay of Flucytosine and Furosemide by High-Pressure Liquid ChromatographyJournal of Pharmaceutical Sciences, 1975