The purification of the proteolytic component of elastase
- 1 October 1959
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 73 (2), 356-361
- https://doi.org/10.1042/bj0730356
Abstract
A practical unit of elastase is defined, and a method of purification, based on acid extraction, fractional dialysis, adsorption and extraction, is described. Electrophoretic examination of each fraction is reported, together with an estimation of the isoelectric point of the final fraction E2I. The isoelectric point of elastase appears to be at pH 8.5, which virtually coincides with the single peak of the pH-activity curve. The mode of variation of specific activity of elastase with initial amount of substrate and enzyme is reported. Most suitable conditions for the definition of elastase unit are discussed. Difficulties due to the complex nature of the elastase system are indicated.Keywords
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