Production of Antibody against Bradykinin: Demonstration of Specificity by Complement Fixation and Radio-Immunoassay

Abstract

The synthesis of a specifically oriented branch-chain copolymer antigen which was able to elicit the production of antibody directed against the polypeptide hormone, bradykinin, is reported. The specificity of the anti-bradykinin antibody is determined in complement fixation studies and in radioactive binding studies. A radio-immunoassay capable of detecting millimicrogram quantities of bradykinin is described. The specificity of the assay is demonstrated by the inability of the polypeptide hormones, vasopression and angiotensin II, to interfere with the binding of labeled bradykinin by specific anti-bradykinin antibody.