Temperature-sensitive virion transcriptase activity in mutants of WSN influenza virus

Abstract
Temperature-sensitive mutants of WSN influenza virus (10, 11, 12) were testedin vitro for activity of the viron RNA-dependent RNA transcriptase at various temperatures. Temperature-sensitivity was found for virion transcriptase activity of mutants beloning to complementation/recombination group I, but not groups II, IV and V. It was not possible on the basis of the results to specify the precise biochemical lesion of mutants from group III.