Isolation of Fibroblast Growth Factor from Bovine Adrenal Gland: Physicochemical and Biological Characterization*
- 1 January 1986
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 118 (1), 82-90
- https://doi.org/10.1210/endo-118-1-82
Abstract
The angiogenic growth factors present in the bovine adrenal gland have been purified by a combination of differential salt precipitation, ion exchange chromatography, and heparin-Sepharose chromatography. They consist of 2 single chain polypeptides with apparent mol wt of 16,000 and 15,000. Sequence analysis of the first 14 residues of both peptides identified the sequences as Pro-Ala-Leu-Pro-Glu-Asp-Gly-Gly- Ser-Gly-Ala-Phe-Pro-Pro for 1 of the peptides and His-Phe- Lys - Asp - Pro - Lys - Arg - Leu - Tyr - x - Lys - Asn - Gly - Gly for the other. The first sequence is identical to residues 1–14 of bovine pituitary and brain fibroblast growth factor (FGF), while the second is identical to residues 1–14 of the corpus luteum (CL) FGF, which is an amino-terminally truncated form of FGF and is otherwise similar, if not identical, to FGF. The biological activity of adrenal FGF is indistinguishable from that of pituitary or brain FGF and CL FGF. They are highly active in triggering the proliferation of culture bovine vascular endothelial cells derived from either large vessels (aortic arch) or CL and adrenal cortex capillaries (half-maximal stimulation at 20–40 pg/ml and saturation at 400–600 pg/ml). In vivo implants containing 50 ng to 1 μg adrenal-derived growth factors stimulate neovascularization in the chorioallantoic membrane of the chick embryo. In addition to being mitogenic for vascular endothelial cells, adrenal FGFs stimulate the proliferation of a wide variety of mesoderm- and neuroectoderm-derived cells, including vascular smooth muscle cells, granulosa and adrenal cortex cells, rabbit costal chondrocytes, and corneal endothelial cells. (Endocrinology118: 82–90, 1986)Keywords
This publication has 15 references indexed in Scilit:
- Isolation and partial molecular characterization of pituitary fibroblast growth factor.Proceedings of the National Academy of Sciences, 1984
- Polypeptide microsequence analysis with the commercially available gas-phase sequencerAnalytical Biochemistry, 1984
- Bovine brain and pituitary fibroblast growth factors: comparison of their abilities to support the proliferation of human and bovine vascular endothelial cells.The Journal of cell biology, 1983
- High-sensitivity amino acid analysis: Methodology for the determination of amino acid compositions with less than 100 picomoles of peptidesAnalytical Biochemistry, 1982
- Demonstration of vasoproliferative activity from mammalian retina.The Journal of cell biology, 1980
- Tumour angiogenesis factor (TAF) and its neutralisation by a xenogeneic antiserumInternational Journal of Cancer, 1979
- Purification of the fibroblast growth factor activity from bovine brain.Journal of Biological Chemistry, 1978
- Production a corpus luteum angiogenic factor responsible for proliferation of capillaries and neovascularization of the corpus luteum.Proceedings of the National Academy of Sciences, 1978
- Demonstration of angiogenesis-activity in the corpus luteum of cattleExperimentelle Pathologie, 1976
- Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent.Proceedings of the National Academy of Sciences, 1976