Chromatography of Human Leukocyte Interferon on Controlled Pore Glass

Abstract

Human leukocyte interferon (HuIFN-.alpha.) was absorbed on controlled pore glass (CPG) beads in 0.01 M potassium phosphate, pH 7.2. Partial recovery of HuIFN-.alpha. from CPG, ranging from 50-75%, was obtained with ethylene glycol (6.8 M), NH4CL (1.0 M) and Tris.cntdot.HCl (1.0 M). A near complete recovery (90%) was obtained with potassium phosphate (1 M) but there was no selectivity in the elution when compared with other proteins. An efficient elution of HuIFN-.alpha. was accomplished with tetramethyl-, and tetraethylammonium chlorides; the recovery of HuIFN-.alpha. was complete at low concentrations (0.25-0.50 M) of these salts at pH 8.0 and the elution of interferon was more selective than with other eluants.