Abstract
Gryllus domesticus was determined using a cryoscopic method in the one drop range in 2 experiments separated by a 6-mon. interval. It is equivalent to -0.73[degree]C. In addition, the tonicities of two representative tissues, testes and muscles, were also assayed after being soaked in Belar''s solution of different osmolalities. The results of these experiments show that testes are probably isotonic with Belar''s solution of the same equivalent osmolallty as hemolymph, (equivalent to 385-387 m0sm KCl) but the isotonic range for muscle is poised at a somewhat higher value (equivalent to 393-413 m0sm KCl).