Heterologous expression of sarcoplasmic reticulum Ca2+-ATPase
- 1 April 1996
- journal article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 16 (2), 107-113
- https://doi.org/10.1007/bf01206200
Abstract
In recent years, expression of rabbit sarcoplasmic reticulum (SR) Ca2+-ATPase in heterologous systems has been a widely used strategy to study altered enzymes generated by site-directed mutagenesis. Various eukaryotic expression systems have been tested, all of them yielding comparable amounts of recombinant protein. However, the relatively low yield of recombinant protein obtained so far suggests that novel purification techniques will be required to allow further characterization of this enzyme based on direct ligand-binding measurements.This publication has 19 references indexed in Scilit:
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