Chemical synthesis and expression in E. coli of a human Val8‐ calcitonin gene by fusion to a synthetic human interferon‐γ gene
- 1 January 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 210 (1), 56-60
- https://doi.org/10.1016/0014-5793(87)81297-8
Abstract
A gene coding for human Val8-calcitonin (Val8-hCT) was synthesized by the solid-phase phosphite approach and fused to a synthetic human immune interferon-γ (IFN-γ) gene. The IFN gene was previously shown to be expressed at a very high level in E. coli [(1986) Gene, in press] due to the control of a strong synthetic promoter and strong ribosome binding site. The cells harboring the fused gene produced 100-150 μg per 1 of bacterial suspension of immunoreactive calcitonin in the form of hybrid IFN-γ-Val8-hCT protein consisting of 140 amino acids. The Val8-hCT can be released from this protein by CNBr treatmentKeywords
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