In VivoMagnetic Resonance Imaging Explorative Study of Ectopic Bone Formation in the Rat
- 1 December 2002
- journal article
- research article
- Published by Mary Ann Liebert Inc in Tissue Engineering
- Vol. 8 (6), 1029-1036
- https://doi.org/10.1089/107632702320934128
Abstract
In animal studies of tissue engineering of bone, histology remains the standard for assessing bone formation. As longitudinal studies with this method are feasible only at the cost of large numbers of animals, we looked for an alternative. Therefore, demineralized bone matrix (DBM) and inactivated demineralized bone matrix (iDBM) implants were subcutaneously implanted in a rat. At 1, 3, 5, and 7 weeks postimplantation soft X-ray and magnetic resonance imaging (MRI) were done to monitor bone formation in the implants. At 7 weeks, the animal was killed and the implants were retrieved for histology. Our results showed that in vivo MRI is well suited to assess bone formation larger than 0.5 mm in diameter and to monitor the complete three-dimensional shape of the newly formed bone noninvasively and longitudinally. The MRI results matched well with the histology results obtained at 7 weeks. In contrast, X-ray imaging appeared inappropriate to monitor the bone formation process in DBM.Keywords
This publication has 17 references indexed in Scilit:
- Donor-Site Complications in Vascularized Bone Flap SurgeryJournal of Investigative Surgery, 2002
- Mandibular reconstruction in miniature pigs with prefabricated vascularized bone grafts using recombinant human osteogenic protein-1: A preliminary studyInternational Journal of Oral & Maxillofacial Surgery, 1999
- Dual-energy X-ray absorptiometry: A reviewJournal of Renal Nutrition, 1999
- Producing Vascularized Bone by Heterotopic Bone Induction and Guided Tissue Regeneration: A Silicone Membrane‐Isolated Latissimus Dorsi Island Flap in a Rat ModelJournal of Reconstructive Microsurgery, 1997
- Accelerated endochondral osteoinduction in the absence of bone matrix particles in a rat model systemJournal of Oral and Maxillofacial Surgery, 1992
- Repair of Calvarial Nonunions by Osteogenin, a Bone-Inductive ProteinPlastic and Reconstructive Surgery, 1990
- A Simple Method for Preparing Thin (10 μm) Histological Sections of Undecalcified Plastic Embedded Bone with ImplantsStain Technology, 1988
- Demineralized Bone ImplantsClinics in Plastic Surgery, 1985
- APPLICATION OF THE BIOLOGICAL PRINCIPLE OF INDUCED OSTEOGENESIS FOR CRANIOFACIAL DEFECTSThe Lancet, 1981
- Bone: Formation by AutoinductionScience, 1965