In Vitro Evaluation of Platelets Stored in CPD‐Adenine Formulations

Abstract

Little information is available about the effect of adenine and added glucose on stored human platelets. Two new formulations, CPDA-2 and CPDA-3 [formulations containing citrate, phosphate, dextrose and adenine] contain 34 mg adenine/63 ml preservative and extra glucose (1.75 and 2.0 .times. the glucose in standard CPD). The in vitro integrity of platelet concentrates stored in CPD, CPDA-1, CPDA-2 and CPDA-3 at 22.degree. C for 72 h was studied. Morphology score, pH, platelet size, population distribution parameters, and EM ultrastructure did not show any adverse effects which could be ascribed to the presence of adenine or extra glucose or both. No differences in platelet ATP concentration or plasma glucose utilization during storage were found between CPD and CPDA-1 platelets. Adenine and added glucose in these preservatives are not detrimental to platelets in vitro by the measures employed.