Monoclonal antibodies to two glycoproteins of herpes simplex virus type 2

Abstract

Monoclonal antibodies to herpes simplex virus type 2 precipitated different numbers of radiolabeled polypeptides from lysates of virus-infected [green monkey kidney Vero] cells. Antibodies directed against 2 viral glycoproteins were characterized. Antibodies from [mouse] hybridoma 17 .alpha.A2 precipitated a 60,000-MW polypeptide which chased into a 60,000- and a 79,000-MW polypeptide. All 3 polypeptides labeled in the presence of [3H]glucosamine and had similar tryptic digest maps. The 60,000-MW polypeptide also chased into a 31,000-MW species which did not label with [3H]glucosamine. Antibodies from [mouse] hybridoma 17.beta.C2 precipitated a 50,000-MW polypeptide which chased into a 56,000- and an 80,000-MW polypeptide. These polypeptides also shared a similar tryptic digest map and labeled with [3H]glucosamine. Both monoclonal antibodies were herpes simplex virus type 2 specific. The viral proteins precipitated by 17.alpha.A2 antibodies had characteristics similar to those reported for glycoprotein E; the proteins precipitated by 17.beta.C2 antibodies appeared to represent a glycoprotein not previously described. This glycoprotein should be tentatively designated glycoprotein F.