The Johnston-Ogston Effect in Sedimenting Proteoglycan Mixtures

Abstract

A simple procedure is formulated to correct for the Johnston-Ogston effect which operates in the velocity sedimentation of mixtures comprising two components. The procedure is applied to the analysis of results obtained with proteoglycan mixtures from bovine nasal cartilage. It is shown that the method permits the evaluation of the true concentration ratio of the components from velocity sedimentation data obtained at two different total concentrations.