Quantitative Immunocytochemical Assay for Infectious Avian Retroviruses
- 1 September 1987
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 68 (9), 2481-2485
- https://doi.org/10.1099/0022-1317-68-9-2481
Abstract
A simple and accurate immunocytochemical focus assay is described, whereby both transforming and non-transforming avian retroviruses can be enumerated. After virus infection of chick embryo fibroblasts, an agar overlay is applied; foci of infected cells (expression foci) are detected immunocytochemically after 5 to 7 days. The primary antibodies are monoclonal sera directed against either viral p19gag or pp60v-src. Detection of expression foci after transfection of cells with cloned viral DNA is also demonstrated.This publication has 6 references indexed in Scilit:
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