Systematic comparison of CRISPR/Cas9 and RNAi screens for essential genes
Top Cited Papers
Open Access
- 9 May 2016
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 34 (6), 634-636
- https://doi.org/10.1038/nbt.3567
Abstract
A side-by-side comparison of CRISPR/Cas9 and RNAi screens for essential genes reveals method-specific differences in performance. We compared the ability of short hairpin RNA (shRNA) and CRISPR/Cas9 screens to identify essential genes in the human chronic myelogenous leukemia cell line K562. We found that the precision of the two libraries in detecting essential genes was similar and that combining data from both screens improved performance. Notably, results from the two screens showed little correlation, which can be partially explained by the identification of distinct essential biological processes with each technology.Keywords
This publication has 40 references indexed in Scilit:
- An integrated encyclopedia of DNA elements in the human genomeNature, 2012
- Saccharomyces Genome Database: the genomics resource of budding yeastNucleic Acids Research, 2011
- Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1Nature, 2009
- Statistical methods for analysis of high-throughput RNA interference screensNature Methods, 2009
- GOrilla: a tool for discovery and visualization of enriched GO terms in ranked gene listsBMC Bioinformatics, 2009
- Highly parallel identification of essential genes in cancer cellsProceedings of the National Academy of Sciences, 2008
- Profiling Essential Genes in Human Mammary Cells by Multiplex RNAi ScreeningScience, 2008
- A probability-based approach for the analysis of large-scale RNAi screensNature Methods, 2007
- Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathwaysNature, 2006
- Expression profiling reveals off-target gene regulation by RNAiNature Biotechnology, 2003