Studies on Conditions for Cell Division and Embryogenesis in Isolated Pollen Culture of Nicotiana rustica
Open Access
- 1 September 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 79 (1), 90-94
- https://doi.org/10.1104/pp.79.1.90
Abstract
A method for the induction of a high rate of cell division and embryogenesis of Nicotiana rustica pollen was developed. Binucleate pollen grains were fractionated by Percoll density gradient (35/45%) centrifugation and cultured in 0.4 molar mannitol at 30°C (the first culture). After 3 days in culture pollen was recollected by a second Percoll fractionation (0/30%) and transferred to and cultured in a medium containing the Murashige-Skoog macro-elements, 0.4 molar mannitol, 40 millimolar galactose, 3 millimolar glutamine, and 5 micromolar ABA for 10 days (the second culture). The cell population consisting of about 80% dividing pollen was transferred to a Murashige-Skoog medium containing 0.4 molar mannitol, 3 millimolar glutamine, and no phytohormone (the third culture), where about 40% of dividing pollen developed into embryos or embryogenic calli.This publication has 4 references indexed in Scilit:
- Factors affecting high-frequency embryo formation inab initio pollen cultures ofNicotianaProtoplasma, 1983
- Selection of embryogenic pollen from cold-treated buds ofNicotiana tabacum var. badischer burley and their development into embryos in culturesProtoplasma, 1980
- High frequency production of embryos inDatura innoxia from isolated pollen grains by combined cold treatment and serial culture of anthers in liquid mediumProtoplasma, 1979
- Pollen Dimorphism—Origin and Significance in Pollen Plant Formation by Anther CultureAnnals of Botany, 1978