Enhanced recovery of solavetivone fromAgrobacterium transformed root cultures ofHyoscyamus muticus using integrated product extraction
- 5 August 1993
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 42 (4), 503-508
- https://doi.org/10.1002/bit.260420414
Abstract
The integrated recovery of solavetivone from fungus elicited “hairy root” cultures of Hyoscyamus muticus is examined using volatile organic solvents and solid-phase adsorbents in an external loop extraction configuration. Hexane and pentane are shown to be toxic when added directly to the culture; however, growth of roots is not inhibited when cultures are exposed to media saturated with these hydrocarbons. Solid-phase neutral adsorbents, XAD-7 and XAD-16, display higher capacity and better solavetivone partitioning capability than the hydrocarbons; however, their selectivity for the sesquiterpene solavetivone is poor in comparison with hexane. In both cases, the integration of product recovery through extraction resulted in a doubling of product formation by alleviating feedback repression. Implications of these results to the recovery of secondary metabolites from plant root cultures are discussed. © 1993 John Wiley & Sons, Inc.Keywords
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