The Identification of Outer Membrane Proteins and Flagella of Campylobacter jejuni

Abstract

The outer membrane proteins of 5 clinical isolates of C. jejuni were identified by 125I-surface labeling and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of outer membrane preparations. All isolates expressed a major outer membrane protein of variable MW 43-46 kilodaltons(K). Several constant surface proteins were also identified including a 27K protein which was surface-epxosed and acid-extractable but was not present in the outer membrane preparations. Isolated flagella comprised a major 62K protein and a minor 87K protein. Both proteins were absent in an flagellate variant. The 62K protein was immunoblotted and immunoprecipitated by rabbit anti-flagella antisera.