Activity of Enzymes Related to Neurotransmission in Neuronal and Glial Fractions
- 1 January 1973
- journal article
- Published by Taylor & Francis in International Journal of Neuroscience
- Vol. 5 (2), 87-90
- https://doi.org/10.3109/00207457309149458
Abstract
The activity of DDC, MAO, ChAc, ChE and AChE was determined in the neuronal, glial and processes fraction isolated from rat brain cortex. The specific activity of DDC was highest in the glial fraction while the specific activity of MAO, ChAc, ChE and AChE was rather evenly distributed in the cell fractions. The results indicate that using the present technique for the bulk isolation of neurons and glia, none of the above enzymes can be localized in one cell fraction only. The results are interpreted as suggesting the presence of DDC, MAO and AChE in glial and neuronal cells.Keywords
This publication has 16 references indexed in Scilit:
- Monoamineoxidase and cholinesterase activity in neurons and neuropil from the rat cerebral cortexJournal of Neurochemistry, 1972
- Dopa-decarboxylase in Autonomic and Sensory Ganglia of the CatActa Physiologica Scandinavica, 1971
- Characterization of Neuronal and Glial Fractions Separated in Sucrose and Ficoll MediaActa Physiologica Scandinavica, 1971
- An improved method for preparing brain cell suspensionsFEBS Letters, 1970
- COMPARATIVE STUDIES ON MITOCHONDRIA ISOLATED FROM NEURON-ENRICHED AND GLIA-ENRICHED FRACTIONS OF RABBIT AND BEEF BRAINThe Journal of cell biology, 1970
- AN ISOTOPIC MICROMETHOD FOR THE MEASUREMENT OF CHOLINESTERASE ACTIVITY IN INDIVIDUAL CELLSJournal of Neurochemistry, 1969
- PROTEIN TURNOVER IN CELL‐ENRICHED FRACTIONS FROM RABBIT BRAIN1Journal of Neurochemistry, 1969
- Monoamine Oxidase in Sympathetic Ganglia of the CatActa Physiologica Scandinavica, 1968
- A Micromethod for the Determination of Choline Acetylase in Individual CellsActa Physiologica Scandinavica, 1967
- A CYTOCHEMICAL STUDY OF THE LOCALIZATION OF CARBONIC ANHYDRASE IN THE NERVOUS SYSTEMJournal of Neurochemistry, 1962