Continuous Counteracting Chromatographic Electrophoresis

Abstract

Counteracting chromatographic electrophoresis (CACE) uses opposing electric and convective fields to focus proteins at the boundary separating distinct granular gel phases. In batch operation, CACE can purify, stack, and concentrate gram quantities of proteins. This technique has been extended to continuous operation by modifying the batch column. Experiments were then used to demonstrate operation of the column. The principles underlying focusing and separation are elucidated by linear chromatography theory.