PARATHYROID HORMONE STIMULATES MAMMALIAN RENAL 25-HYDROXYVITAMIN D3-1α-HYDROXYLASE IN VITRO
- 1 January 1982
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 110 (1), 294-296
- https://doi.org/10.1210/endo-110-1-294
Abstract
We have shown that parathyroid hormone (PTH) stimulates 25-hydroxyvitamin D3-1α-hydroxylase activity in mitochondrial preparations of guinea pig kidney in vitro.1,25-[3H]dihydroxyvitamin D3 produced from 25-[3H]hydroxyvitamin D3 was identified by both normal phase and reversed-phase high pressure liquid chromatography. Enzyme stimulation by PTH was dose-dependent, peaked after 15 minutes of incubation with hormone and was maximal at concentrations of approximately 10-10 M PTH-(1–84) and PTH-(1–34). This guinea pig renal model therefore permits direct examination of hormonal regulation of the mammalian enzyme in vitro.Keywords
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