Evidence that ethylenediamine acts in the isolated ileum of the guinea-pig by releasing endogenous GABA

Abstract

1 Ethylenediamine (EDA) released [³H]-γ-aminobutyric acid ([³H]-GABA) in a dose-dependent manner from the isolated preloaded ileum of the guinea-pig maintained in Krebs-bicarbonate solution (pH 7.4, 37°C), in the presence of β-alanine and amino-oxyacetic acid (AOAA) to prevent GABA uptake into glial cells and catabolism. This release was reversibly prevented by 3-mercaptopropionic acid (3-MPA), also in a dose-dependent manner. 2 In the isolated ileal preparations of the guinea-pig maintained in Krebs-bicarbonate solution, EDA induced a dose-dependent transient, cholinergic contractile response (GABA_A-receptor-mediated effect), followed by an ‘after-relaxation’ (GABA_B-receptor-mediated effect). EDA also induced a transient contraction superimposed on repetitive twitch responses to electrical transmural stimulation of the cholinergic neurones, followed by a depression of the twitch contractions. 3 This GABA_A-receptor-mediated contraction was antagonized by bicuculline methochloride and picrotoxinin, whilst the GABA_B-receptor-mediated ‘after-relaxation’, and depression of cholinergic twitch contractions, was susceptible to antagonism by δ-aminovaleric acid. The pA₂ value for bicuculline methochloride antagonism of EDA was estimated to be 5.8, identical with that for GABA. 4 3-Mercaptopropionic acid also prevented these pharmacological actions induced by EDA without affecting responses to GABA, 3-aminopropranesulphonic acid, muscimol, baclofen or the twitch responses to transmural stimulation. 5 It is concluded that EDA releases both [³H]-GABA and endogenous GABA in the guinea-pig ileum, thus providing further evidence that GABA is a transmitter in the enteric nervous system.