Properties, Potentials, and Prospects of Antifreeze Proteins
- 1 January 2008
- journal article
- review article
- Published by Taylor & Francis in Critical Reviews in Biotechnology
- Vol. 28 (1), 57-82
- https://doi.org/10.1080/07388550801891152
Abstract
Antifreeze proteins (AFPs) are a group of proteins that protect organisms from deep freezing temperatures and are expressed in vertebrates, invertebrates, plants, bacteria, and fungi. The nuclear magnetic resonance, x-ray structure, and many spectroscopic studies with AFPs have been instrumental in determining the structure–function relationship. Mutational studies have indicated the importance of hydrophobic residues in ice binding. Various studies have pointed out that the mechanism of AFP action is through its adsorption on the ice surface, which leads to a curved surface, preventing further growth of ice by the “Kelvin effect.” The AFPs have potential industrial, medical, and agricultural application in different fields, such as food technology, preservation of cell lines, organs, cryosurgery, and cold hardy transgenic plants and animals. However, the applications of AFPs are marred by high cost due to low yield. This review deals with the source and properties of AFPs from an angle of their application and their potential. The possibility of production using different molecular biological techniques, which will help increase the yield, is also dealt with.Keywords
This publication has 267 references indexed in Scilit:
- Microinjection of the antifreeze protein type III (AFPIII) in turbot (Scophthalmus maximus) embryos: Toxicity and protein distributionAquaculture, 2006
- A part of ice nucleation protein exhibits the ice‐binding abilityFEBS Letters, 2005
- Enhanced expression of human cytochrome P450 1A2 by co-expression with human molecular chaperone Hsp70Toxicology Letters, 2004
- Understanding the mechanism of ice binding by type III antifreeze proteinsJournal of Molecular Biology, 2001
- Cryopreservation of mammalian embryos and oocytes: Recent advancesBiotechnology Advances, 1996
- Expression of a cystine-rich fish antifreeze in transgenicDrosophila melanogasterTransgenic Research, 1996
- Antifreeze proteins and their potential use in frozen foodsBiotechnology Advances, 1995
- The effects of antifreeze proteins on chilled and frozen meatMeat Science, 1994
- Hypothermic protection — A fundamental property of “Antifreeze” proteinsBiochemical and Biophysical Research Communications, 1991
- Antifreeze proteins from the ocean pout, Macrozoarces americanus: circular dichroism spectral studies on the native and denatured statesBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1986