Casein and Factor 3 in Dietary Necrotic Liver Degeneration; Concentration of Factor 3 from Casein

Abstract

Summary 1. Samples of crude and “vit. Free,” alcohol-extracted casein, and also enzymatic casein digests, were found to contain considerable amounts of factor 3, which protects against dietary necrotic liver degeneration. This explains why the deficiency does not develop on customary vit. E free casein diets. 2. A method is described for the concentration of factor 3 from an enzymatic casein hydrolysate, affording a 30-fold purification in 3 steps, with an over-all recovery of about 38%. This concentrate is being used for the further purification of factor 3.