Evidence for the Association of 2′,3′‐Cyclic‐Nucleotide 3′‐Phosphodiesterase with Myelin‐Related Membranes in Peripheral Nervous System

Abstract

In the rabbit PNS [peripheral nervous system] the specific activity of 2'',3''-cyclic nucleotide 3''-phosphodiesterase (CNP) in myelin was not enriched over the starting homogenate. Most of the total activity was recovered in myelin. In myelin-deficient mutants, low CNP activities were measured in sciatic nerves. CNP specific activities were similar in myelinated and non-myelinated nerves. In non-nervous tissues they were significantly lower than in nervous tissue. Isoenzymes of CNP were not evident in peripheral nerves. CNP apparently is present in PNS myelin and preferentially localized in Schwann cell plasma membranes.