A REDOR NMR Study of a Phosphorylated Statherin Fragment Bound to Hydroxyapatite Crystals
- 9 June 2005
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 127 (26), 9350-9351
- https://doi.org/10.1021/ja050910m
Abstract
Hydroxyapatite (HAP) is the main mineral component of teeth. It is well-known that several salivary proteins and peptides bind strongly to HAP to regulate crystal growth. Interactions between a peptide derived from the N-terminal fragment of the salivary protein statherin and HAP were measured utilizing rotational-echo double-resonance (REDOR) nuclear magnetic resonance (NMR). The REDOR measurement from the side chain of the salivary peptide to the HAP surface is complicated by two effects: a possible additional dipolar coupling to a phosphorylated side chain and the potential proximity of phosphorus atoms to each other, resulting in a homonuclear dipolar interaction. Both of these effects were addressed, and the smallest model applicable to our system includes the nitrogen-15 (15N) spin in the lysine side chain and two phosphorus-31 (31P) spins, at least one of which must be from the surface phosphates of the HAP.Keywords
This publication has 12 references indexed in Scilit:
- Measurement of 13C−15N Distances in Uniformly 13C Labeled Biomolecules: J-Decoupled REDORJournal of the American Chemical Society, 1999
- REDOR-Determined Distances from Heterospins to Clusters of13C LabelsJournal of Magnetic Resonance, 1999
- Structural Features of Protein−Nucleic Acid Recognition SitesBiochemistry, 1999
- Delineation of Conformational Preferences in Human Salivary Statherin by1H,31P NMR and CD Studies: Sequential Assignment and Structure-Function CorrelationsJournal of Biomolecular Structure and Dynamics, 1998
- REDOR Dephasing by Multiple Spins in the Presence of Molecular MotionJournal of Magnetic Resonance, 1997
- The REDOR transform: direct calculation of internuclear couplings from dipolar-dephasing NMR dataChemical Physics Letters, 1995
- A simple magic angle spinning NMR experiment for the dephasing of rotational echoes of dipolar coupled homonuclear spin pairsChemical Physics Letters, 1992
- Inhibition of calcium phosphate precipitation by human salivary statherin: Structure-activity relationshipsCalcified Tissue International, 1992
- Detection of Weak Heteronuclear Dipolar Coupling by Rotational-Echo Double-Resonance Nuclear Magnetic ResonancePublished by Elsevier ,1989
- Lysine—phosphate hydrogen bonds and hydrogen-bonded chains with large proton polarizability in polylysine—phosphate systems: ir investigationsJournal of Molecular Structure, 1986