In vitro synthesis of an infectious RNA from cDNA clones of human rhinovirus type 14
- 1 November 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 56 (2), 628-632
- https://doi.org/10.1128/jvi.56.2.628-632.1985
Abstract
Development of a novel infectious cDNA assay is described from human rhinovirus type 14. A full-length cDNA clone of the human rhinovirus type 14 genome RNA was assembled and transcribed in vitro by using the SP6 transcription system. Transfection of HeLa cells with the nascent RNA resulted in the production of rhinovirus indistinguishable from the parental virus by both immunological and polyacrylamide gel analysis.This publication has 17 references indexed in Scilit:
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