Interaction and functional collaboration of p300/CBP and bHLH proteins in muscle and B-cell differentiation.
- 1 October 1996
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 10 (19), 2478-2490
- https://doi.org/10.1101/gad.10.19.2478
Abstract
Differentiation of skeletal muscle cells and B lymphocytes is regulated by basic helix-loop-helix (bHLH) proteins. Both differentiation programs are inhibited by the adenovirus E1A oncoprotein. Analysis of E1A mutants has implicated two of its cellular-binding proteins, p300 and CBP, in controlling certain aspects of differentiation. We find that p300 can cooperate with tissue-specific bHLH proteins in activating target genes and requires only the bHLH domain of such proteins to stimulate E box-directed transcription. Importantly, the ability of bHLH proteins to activate transcription correlates with the presence of p300/CBP in E box-dependent DNA-binding complexes, because both phenomena require at least two adjacent E-box motifs. Microinjection of p300/CBP antibodies into myoblasts blocks terminal differentiation, cell fusion, and transcriptional activity of myogenic bHLH proteins. These results suggest that the function of p300/CBP is essential for the execution of key aspects of cellular differentiation.Keywords
This publication has 50 references indexed in Scilit:
- A CBP Integrator Complex Mediates Transcriptional Activation and AP-1 Inhibition by Nuclear ReceptorsCell, 1996
- Absence of blood formation in mice lacking the T-cell leukaemia oncoprotein tal-1/SCLNature, 1995
- Activation of cAMP and mitogen responsive genes relies on a common nuclear factorNature, 1994
- Phosphorylated CREB binds specifically to the nuclear protein CBPNature, 1993
- Myogenin gene disruption results in perinatal lethality because of severe muscle defectNature, 1993
- Muscle deficiency and neonatal death in mice with a targeted mutation in the myogenin geneNature, 1993
- Helix-loop-helix transcription factor E47 activates germ-line immunoglobulin heavy-chain gene transcription and rearrangement in a pre-T-cell line.Genes & Development, 1991
- MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancerCell, 1989
- Adenovirus E1A products suppress myogenic differentiation and inhibit transcription from muscle-specific promotersNature, 1988
- Adenovirus-2 E1A products repress enhancer-induced stimulation of transcriptionNature, 1984