GLYCOPEPTIDES FROM SURFACE OF HUMAN NEUROBLASTOMA-CELLS

Abstract

Glycopeptides suggesting a complex oligosaccharide composition are present on the surface of cells from human neuroblastoma tumors and several cell lines derived from the tumors. The glycopeptides, labeled with radioactive L-fucose, were removed from the cell surface with trypsin, digested with pronase and examined by chromatography on Sephadex G-50. Human skin fibroblasts, brain cells and a fibroblast line derived from neuroblastoma tissue show less complex glycopeptides. Although some differences exist between cell lines and primary tumor cells, the similarities between these human tumors and animal tumors examined previously are striking.