Molecular Characterization of Oat Seed Globulins
- 1 July 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 87 (3), 698-704
- https://doi.org/10.1104/pp.87.3.698
Abstract
We have isolated full-length clones that encode oat (Avena sativa) seed storage globulin mRNAs from a DNA library in the expression vector lambda gtll. The longest of these clones, pOG2, has an 1840-base pair insert that encodes a complete precursor subunit with a signal peptide of 24 amino acids followed by an acidic polypeptide of 293 amino acids and a basic polypeptide of 201 amino acids. Near the C terminus of the acidic polypeptide are four repeats of a highly conserved, glutamine-rich octapeptide. Other oat globulin cDNA clones contain five of these repeats. Nucleotide sequence comparisons between these clones indicate that the genes encoding these proteins are highly conserved. We estimate there to be 7 to 10 gene for the oat globulin haploid genome. Comparisons of amino acid sequences show that the oat globulin is 30 to 40% homologous with storage globulins of legumes and about 70% homologous with the rice seed storage globulin (glutelin).This publication has 22 references indexed in Scilit:
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