Soluble factors for B cell activation: a T cell hybridoma that secretes a specific B cell growth factor.
- 1 February 1983
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 130 (2), 777-780
- https://doi.org/10.4049/jimmunol.130.2.777
Abstract
A murine T cell hybridoma, T91, has been established by fusion of BALB/c Con A-activated spleen cells with BW 5147 HAT-sensitive thymoma cells. T91 constitutively produced a factor that markedly enhanced the proliferative response to LPS of B cells cultured at low cell density. This factor is nonmitogenic by itself. It maintains in the absence of LPS, the proliferation of B cell blasts previously activated with LPS for 3 days. T91 supernatant contains neither IL 1 nor IL 2 nor TRF activity, and mixing experiments indicate the absence of factors suppressing these activities. The data suggest the existence of a specific B cell growth factor compatible with a two-signal model for B lymphocyte activation.This publication has 1 reference indexed in Scilit: