Cytopathology, plaque assay, and heat inactivation of hepatitis A virus strain HM175
- 1 May 1987
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 22 (1), 35-44
- https://doi.org/10.1002/jmv.1890220106
Abstract
Hepatitis A virus (HAV) strain HM175 derived after repeated subculture of persistently infected B-SC-I cells caused a specific cytopathic effect upon acute infection of B-SC-1 cells. The virus formed visible plaques on B-SC-I cell monolayers after 9 to 14 days of incubation at 34°C. and virus can therefore be titrated by plaque assay. Virus could be re-isolated from plaques of infected cells. which allows the clonal isolation of HAV variants. The stability of a plaquepurified variant of HAV at elevated temperatures exceeded that of poliovirus type 1. but this variant is less stable than previously reported strains of HAV.Keywords
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