Immunization against Plasmodium falciparum with recombinant polypeptides produced in Escherichia coli
- 1 November 1988
- journal article
- research article
- Published by Wiley in Parasite Immunology
- Vol. 10 (6), 607-617
- https://doi.org/10.1111/j.1365-3024.1988.tb00248.x
Abstract
Summary Two proteins produced in recombinant Escherichia coli and containing ammo acid sequences from the Plasmodium falciparum precursor to major merozoite surface antigens (PMMSA) have been partially purified. These proteins, together with a preparation of merozoites, have been used to immunize animals. The antibody response and the degree of protection were compared. Animals immunized with merozoites produced antibodies reacting with many P. falciparum proteins. whereas a response specific for PMMSA was detected in those receiving the recombinant material. Incomplete protection was conferred to both groups and there was no apparent correlation between antibody levels and protection.Keywords
This publication has 18 references indexed in Scilit:
- Serum from Pf195 protected Aotus monkeys inhibit Plasmodium falciparum growth in vitroExperimental Parasitology, 1987
- Induction of protective immunity against experimental infection with malaria using synthetic peptidesNature, 1987
- Immunization with synthetic peptides of a Plasmodium falciparum surface antigen induces antimerozoite antibodies.Proceedings of the National Academy of Sciences, 1986
- Immunization of Aotus monkeys with recombinant proteins of an erythrocyte surface antigen of Plasmodium falciparumNature, 1986
- Primary structure of the precursor to the three major surface antigens of Plasmodium falciparum merozoitesNature, 1985
- Major surface antigen gene of a human malaria parasite cloned and expressed in bacteriaNature, 1984
- The three major antigens on the surface of Plasmodium falciparum merozoites are derived from a single high molecular weight precursor.The Journal of Experimental Medicine, 1984
- Surface antigens of malaria merozoites. A high molecular weight precursor is processed to an 83,000 mol wt form expressed on the surface of Plasmodium falciparum merozoites.The Journal of Experimental Medicine, 1983
- Biosynthesis and processing of a Plasmodium falciparum schizont antigen recognized by immune serum and a monoclonal antibody.The Journal of Experimental Medicine, 1982
- MEROZOITE VACCINATION OF DOUROUCOULI MONKEYS AGAINST FALCIPARUM MALARIAThe Lancet, 1977