Evaluation of the relationship between interleukin‐1 gene cluster polymorphisms and early implant failure in non‐smoking patients
- 9 December 2004
- journal article
- Published by Wiley in Clinical Oral Implants Research
- Vol. 16 (2), 194-201
- https://doi.org/10.1111/j.1600-0501.2004.01091.x
Abstract
The aim of the present study was to investigate the relationship between specific polymorphisms of the interleukin-1 gene cluster and the early failure of osseointegrated implants. The subject population was composed by a test group comprising 28 non-smoking patients (mean age 52.7) that had suffered one or more early implant failures and by a control group consisting of 34 individuals (mean age 43.3) with one or more healthy implants. Genomic DNA from buccal mucosa was amplified by the polymerase chain reaction (PCR), followed by restriction fragment length polymorphism (RFLP) and submitted to polyacrylamide gel electrophoresis to distinguish the alleles of the interleukin-1A (-889), interleukin-1B (+3953), interleukin-1B (-511) and interleukin-RN (intron 2) gene polymorphisms. Differences in the allele and genotype frequencies between control and test groups were assessed by chi(2) test or by Monte Carlo simulations (P<0.05). Haplotype frequencies, linkage disequilibrium and Hardy-Weinberg equilibrium were also estimated. No statistically significant differences were found in the genotype distribution or allelic frequencies of the polymorphisms. No differences were observed between control and test groups when different interleukin-1 gene cluster haplotypes were compared. Nevertheless, the interleukin-1A (-889) and interleukin-1B (+3953) polymorphic sites were in strong linkage disequilibrium (P=0.00014 for control group and P=0.0238 for the test group). This study suggests that polymorphisms in the interleukin-1 gene cluster are not associated with early implant failure in a non-smoking Brazilian population.Keywords
This publication has 46 references indexed in Scilit:
- A Critical Assessment of Interleukin‐1 (IL‐1) Genotyping When Used in a Genetic Susceptibility Test for Severe Chronic PeriodontitisThe Journal of Periodontology, 2002
- Do interleukin-1 polymorphisms predict the development of jreiodontitis or the success of dental implants?Journal of Periodontal Research, 2002
- Genetic Polymorphisms of the IL‐1α and IL‐1β Genes in African‐American LJP Patients and an African‐American Control PopulationThe Journal of Periodontology, 2000
- The Relationship Between the Interleukin– 1 Periodontal Genotype and Implant Loss. Initial DataThe Journal of Periodontology, 1999
- The Influence of Interleukin Gene Polymorphism on Expression of Interleukin–1β and Tumor Necrosis Factor–α in Periodontal Tissue and Gingival Crevicular FluidThe Journal of Periodontology, 1999
- Linkage Disequilibrium of Interleukin‐1 Genetic Polymorphisms With Early‐Onset PeriodontitisThe Journal of Periodontology, 1999
- Monte Carlo tests for associations between disease and alleles at highly polymorphic lociAnnals of Human Genetics, 1995
- lnterleukin-1 Receptor AntagonistCritical Reviews in Immunology, 1995
- An Assessment of Survival Rates and Within-patient Clustering of Failures for Endosseous Oral ImplantsJournal of Dental Research, 1993
- Periodontal tissues and their counterparts around endosseous implantsClinical Oral Implants Research, 1991