Combined radiolabel-binding and immunocytochemical evaluation of receptor–ligand interactions. Studies of transferrin receptors on activated lymphocytes

Abstract

A protocol that involved both immunohistological and radiolabel-binding procedures was devised for the study of transferrin-receptor interactions in human lymphocytes. This composite approach yielded considerably more information than did either technique used alone, and also provided a simple means for exclusion of several common potential sources of error.