The analysis ofCis‐trans fatty acid isomers using gas‐liquid chromatography

Abstract

The geometric isomers of many unsaturated fatty acid methyl esters can be separated using high‐resolution gas‐liquid chromatography on polyester or Apiezon columns. Separations reported for the geometric isomers of monounsaturated, ricinoleic, linoleic, conjugated, and epoxy fatty acids are reviewed here. New data is presented on the resolution of linolenate geometric isomers on both polyester and Apiezon columns. The separation of methyl oleate and methyl elaidate on a polyester column has also been accomplished. Techniques for preparing and using the high‐resolution columns necessary for these separations are reviewed.