Determination of triamcinolone acetonide in cream and suspension formulations by high-performance liquid chromatography

Abstract

Methods are described for the determination of triamcinolone acetonide at concentrations of 0.025 and 0.1%m/m in creams and 2 and 6 mg ml^–1 in suspensions. Triamcinolone acetonide extracted from these formulations is assayed by high-performance liquid chromatography, using a reversed-phase separation on a Spherisorb S10 ODS column with a solvent system of methanol and water. Prednisolone is used as an internal standard. The main advantages of the method are the over-all speed of analysis and the degree of precision obtained.