Primary in vitro cytotoxic T cell response to non-major histocompatibility complex alloantigens in normal mice.
Open Access
- 1 August 1982
- journal article
- research article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 156 (2), 610-621
- https://doi.org/10.1084/jem.156.2.610
Abstract
It is possible to consistently generate a primary in vitro cytotoxic T cell (Tc) response to non-major histocompatibility complex alloantigens using responder cells from a normal mouse strain. This is achieved by carrying out, in the generating phase, a limiting dilution procedure in which it appears that suppressor cells that inhibit Tc activation or expansion are too dilute to manifest their effect. The response was observed in mouse serum (MS)- and fetal calf serum (FCS)-supplemented media, an important finding in the light of the anomalous nonspecific effect induced by FCS. The cytotoxic response produced in MS-supplemented media was highly specific in both the generating and effector phases, whereas the responses in FCS had a strong nonspecific component.This publication has 25 references indexed in Scilit:
- Mouse T Cell-Mediated Cytolysis Specifically Triggered by Cytophilic Xenogeneic Serum Determinants: A Caveat for the Interpretation of Experiments Done under “Syngeneic” ConditionsThe Journal of Immunology, 1978
- Primary in vitro cell-mediated lympholysis reaction of NZB mice against unmodified targets syngeneic at the major histocompatibility complex.The Journal of Experimental Medicine, 1978
- Quantitative Studies of the Activation of Cytotoxic Lymphocyte Precursor CellsImmunological Reviews, 1977
- Secondary in vitro responses of T lymphocytes to non-H-2 alloantigens self-H-2-restricted responses induced in heterologous serum are not dependent on primary-stimulating non-H-2 alloantigens.The Journal of Experimental Medicine, 1977
- Heterologous Sera: A Target for in Vitro Cell-Mediated CytotoxicityThe Journal of Immunology, 1976
- Interaction antigens detected by cytotoxic T cells with the major histocompatibility complex as modifierNature, 1975
- In vitro cell-mediated immune responses to the male specific(H-Y) antigen in mice.The Journal of Experimental Medicine, 1975
- Quantitative analysis of the 51Cr release cytotoxicity assay for cytotoxic lymphocytesCellular Immunology, 1974
- A miniaturized mouse mixed leukocyte culture in serum-free and mouse serum supplemented mediaJournal of Immunological Methods, 1973
- Immunogenetic and Biological Aspects of in Vitro Lymphocyte Allotransformation (MLR) in the MouseImmunological Reviews, 1973