Bile Acid Synthesis in Man: Metabolism of 7α-Hydroxycholesterol-14C and 26-Hydroxycholesterol-3H
Open Access
- 1 January 1972
- journal article
- Published by American Society for Clinical Investigation in Journal of Clinical Investigation
- Vol. 51 (1), 112-117
- https://doi.org/10.1172/jci106780
Abstract
The pathways of bile acid synthesis in man were evaluated by studying the metabolism of 7α-hydroxycholesterol-4-14C and 26-hydroxycholesterol-16, 22-3H administered parenterally to individuals requiring external biliary drainage. Techniques for the identification of metabolites were thin-layer chromatography, column chromatography, gas-liquid chromatography with stream splitting, and crystallization to constant specific activity. It was found that both compounds were rapidly metabolized to bile acids and excreted in bile. Of the total radioactivity recovered in bile as bile acids, 87% of the 26-hydroxycholesterol-3H and 90% of the 7α-hydroxycholesterol-14C was found to be metabolized to both chenodeoxycholate and cholate. Compared to 7α-hydroxycholesterol, a greater proportion of 26-hydroxycholesterol was found to be metabolized to chenodeoxycholate. These findings indicate that both 7α-hydroxycholesterol and 26-hydroxycholesterol can be intermediates in the metabolism of cholesterol to bile acids in man. The observation that conversion to cholate takes place less readily after C-26 hydroxylation is consistent with previous findings in other species.This publication has 19 references indexed in Scilit:
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