An efficient synthetic primer for the M13 cloning dideoxy sequencing system
Open Access
- 1 September 1982
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 2 (9), 687-696
- https://doi.org/10.1007/bf01114830
Abstract
The deoxytetradecamer d(AAAACGACGGCCAG) is an excellent universal primer for sequence determination of DNA cloned into the phage M13 mp7, mp8 and mp9 series. This new primer offers several advantages over others currently available and it is used to define the cloning of Hinf I fragments of S13 DNA into the EcoRI site of M13 mp7, utilizing the homologous complementary base pairing of the 2 restriction sites. Of the 4 possible sequence derivatives of the Hinf I GANTC recognition site, only those corresponding to GAATC and GATTC were found at cloning sites in chimeras.This publication has 20 references indexed in Scilit:
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